SFXN5 Knockout cell line (Y79)

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location： Home > Products > KO Cell Lines > SFXN5 Knockout cell line (Y79)

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SFXN5 Knockout cell line (Y79) Order

SFXN5 Knockout cell line (Y79)
Catalog#
YKO-H1097
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SFXN5 Knockout cell line (Y79)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SFXN5 Knockout cell line（Y79）
Cell Morphology
Grow in clumps, suspension
Passage ratio
1：2-1：3
Species
Human
Gene Name
SFXN5
Gene ID
94097
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80%RPMI-1640+20%FBS
Accession
CVCL_E1F7(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript SFXN5-201)
Validation: PCR+Sanger Sequencing
SFXN5 Knockout cell line (Y79) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Y79		 STR Info (Cell bank)
Cell Line:Y79
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 8989
D16S539 13131314
VWA 15181518
TH01 6666
AMEL XXXX
TPOX 8888
CSF1PO 11121112
D12S391 2124
FGA 2022
D2S1338 2020
D21S11 303132.0
D18S51 1316
D8S1179 1316
D3S1358 1516
D6S1043 1218
PENTAE 1318
D19S433 1313
PENTAD 1212
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SFXN5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Y79 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SFXN5 gene knockout SFXN5 Knockout cell line（Y79） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SFXN5 Knockout cell line (Y79)
SFXN5 Knockout cell line (Y79)
Catalog#
YKO-H1097
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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